DNA extraction of blood samples were performed under standardized techniques of Dynabeads ® DNA DIRECT ™.
In addition, scoring method of periodontal disease index (PDI) of Ramfjord for diagnosis of periodontitis was achieved.ĭNA extraction: Peripheral whole blood (5 ml) with anti-coagulant (EDTA) tube was collected from each of individual. Each patient was checked clinically by using of case history analysis recording, an evaluation of clinical examination, various diagnostic aids such as radiographs and laboratory investigations.Īll patients were examined using measurement of probing depth, clinical attachment loss, bone resorption, and tooth mobility. Total subjects participated in the study were 114 (60 patients with chronic periodontitis and 54 controls without chronic periodontitis).Ĭlinical risk scores of periodontal diseaseĭiagnosis of periodontal disease was performed by detection of any pathological condition that affecting tooth supporting structures. Patient and control subjects coming for routine follow up and were asked to participate in the research. The study was conducted at Tripoli Central Hospital. Īdditional study was determined the susceptibility of people to extra destructive process by using genetic and immunological response, which was evaluated as 10-15% and recurrent among members of the same family.
įurther studies were revealed that periodontitis was dominant and associated with different parameters such as male gender, Mexican race, low education, current smokers, diabetes, stress, crooked or crowded teeth, medications that cause dry mouth, immunedeficiencies, hormonal changes in women. The study that performed by 3rd national health and nutrition examination survey (NHANES III) (1999-2004) was revealed that 8.52% of American adults between age 20 to 64 have periodontal disease. The distribution of periodontal diseases among all populations were small percentage (10-15%). IntroductionĬhronic periodontal disease is a variable from simple gingival disease to aggressive and necrotizing general periodontitis. Generally, there is non-significant differences were observed in HLA-DRB3, HLA-DRB4Ĭonclusion: The HLA-B45 and HLA-A30 alleles may represent as risk factors for chronic periodontitis cases in Libyan population, whereas HLA-DRB114 and HLA-DRB5 may indicate to protective factors for chronic periodontitis among Libyan populations. Furthermore, statistically significant differences were observed between patients and controls in HLA-DRB114 and HLA-DRB5 alleles, P<0.00 and P<0.00, respectively. Results: Distribution of different HLA-B and HLA-A loci among patients and control individuals were statistically significant in the HLA-B45 and HLA-A30 alleles, P<0.03 and P<0.04, respectively. All study population was examined clinically using Ramfjord chart for diagnosis the periodontitis cases and subsequently were analyzed their blood samples using specific-sequence oligonucleotide PCR (SSO-PCR) assay for typing various HLA loci. Material and methods: A total of 114 individuals (60 patients with chronic periodontitis and 54 controls) were collected from Tripoli Central Hospital. This study was performed to find out the association between HLA classes I and II genes with chronic Human leukocyte antigen (HLA) gene has an important role in determinant the susceptibility and progression of periodontitis in human to facilitate diagnostic and therapeutic value.
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View PDF Download PDF Tables & Figures Abstractīackground: Periodontitis is an inflammation of gums and teeth bone that caused by certain bacterial normal flora in the mouth leading to destroy periodontal tissues. Visit for more related articles at Journal of Infectious Diseases & Therapy
Related article at Pubmed, Scholar Google This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: JAccepted: SeptemPublished: September 22, 2015Ĭitation: Ben-Darif E, Daeki AO, Maroof F (2015) The Role of Human Leukocyte Antigen Typing in Libyan Patients with Chronic Periodontitis. The Role of Human Leukocyte Antigen Typing in Libyan Patients with Chronic Periodontitis Ben-Darif E 1 *, Daeki AO 2 and Maroof F 2ġ Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli, LibyaĢ Department of Medical Sciences, Libyan Academy of higher studies, Tripoli, Libyaĭepartment of Medical Microbiology and Immunology
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